Esperance suffered a 2-0 defeat to Flamengo in their opening 2025 FIFA Club World Cup match in Philadelphia.
Giorgian de Arrascaeta and Luís Araújo scored in each half, securing a win for the Copa Libertadores holders.
This result puts Flamengo top of Group D alongside Chelsea after the first round.
The loss puts pressure on Esperance, who need a positive result against Los Angeles FC in their next match to keep their qualification hopes alive.
The North African side are looking to bounce back after being defeated in their return to global competition.